Dr. Richard S. Lord, nutritional biochemist extraordinaire and Chief Science Officer at Metametrix Clinical Laboratory (now Genova) recently retired after 25 years. Richard was the director of the Medical Education team when I was in Atlanta at the lab. Richard was and continues to be, my teacher. There has been no greater influence on my thinking as a clinician and scientist than Dr. Lord. He inspired me to always question, embrace the uncertainty, trust the data, and argue my point (with evidence, of course!) RSL, this blog is for you!
When I interviewed for the postdoctorate position at Metametrix Clinical Laboratory in 2004, I was a bright-eyed student finishing my final year in naturopathic medical school. I was nervous and excited. The lab was a marvel of glass and metal. Gleaming rows of instruments: LC MS/MS, GC/MS, HPLCs and on…. Lots of machines that go “bing” as Monty Python said.
There was a library with thousands of journals and almost as many textbooks - and a librarian, Cathy Morris, dedicated to obtaining full text article requests.
I was in awe.
My interview with Dr. Lord that day was not unlike skimming along the white tips of ocean waves on a speed boat. I may have only caught every fifth concept that he expounded on but I was transfixed. There was no other place on earth I wanted to be!
Richard’s focus in that 2004 interview and for some months thereafter was around the clinical implications of low plasma homocysteine. He was gracious in allowing me to participate in writing the company’s white paper introducing the concept.
I know, you’re a good integrative clinician, and low plasma homocysteine is old hat to you at this point. But at the time, virtually no one was thinking about its relevance as an essential precursor to glutathione, taurine and sulfate; that low levels of homocysteine suggested that the body’s ability to respond to oxidative stress with glutathione and to engage in phase II biotransformation, was at serious risk.
If we thought about homocysteine at all, it was as a cardiovascular risk marker, where only high levels were considered relevant.
Now, transmethylation and transsulfuration are key pathways memorized by all students of integrative and functional medicine. Methylated folate, B12, n-acetyl cysteine are prescribed routinely.
Dr. Richard Lord put low homocysteine on the map and educated us as to its relevance. Quest Diagnostics later adopted a lower limit after Metametrix started reporting it.
From Laboratory Evaluations in Integrative and Functional Medicine, Bralley and Lord, 2008
I was moved by the magic, the power, of nutritional biochemistry and the intellectual journeys I took with Richard. It seemed to me then--and it still does today--that the solution is present in understanding as fully as possible the question.
We could look “under the biochemical hood” of the individual, piece together their metabolic story and correct it with pinpointed nutritional and dietary interventions.
And it worked!
We could effectively reduce disease risk, oxidative stress, and inflammation; improve mitochondrial function, energy, mood, sleep; identify and reduce toxic burden and reverse neurological deficits and tweak the gut microbiome. Sometimes, we could turn the disease process around completely, uncover and successfully treat genetic conditions otherwise missed. Nothing was outside our reach of investigation. It was a grail quest.
I remember consulting with a doctor regarding a patient’s organic acid results. This individual had a seizure disorder, onset at 16, which was non-responsive to any medication.
Her organic acids revealed a very elevated beta hydroxyisovalerate (BHIV), a catabolic intermediate compound derived from the amino acid valine that accumulates with a biotin deficiency. In her case, given the severity of her condition, there was likely a mutation in the biotin-dependent carboxylase enzymes required to metabolize BHIV. The mutation wasn’t apparent at birth, when organic acids are routinely tested, looking for inborn errors of metabolism. It was too mild to be seen with the broad reference ranges able to identify only the severest cases. She wasn’t symptomatic until 16 years of age. It took the more sensitive reference ranges we employed at the lab to identify it.
The young woman was treated safely with high doses of biotin, which pushed the faulty carboxylase enzymes to function. And her seizures resolved.
Another great case involved a young man with Melnick-Needles Syndrome. MNS is an extremely rare condition caused by a mutation in the gene that codes for filamin A, a protein that provides structure for cells. Being such a fundamental player in sustaining life, a filamin A mutation is often fatal shortly after birth.
We carefully individualized his nutrient intake of amino acids, fatty acids, vitamins, minerals to meet his exact needs by looking at his laboratory data every six months. It worked for him.
At the time of my last consultation regarding this patient, he was graduating high school. He was the oldest known living individual with MNS.
These are the sexy, current terms used to describe what was started at Metametrix in 1984, when Dr. Andy Bralley began measuring plasma amino acids - the first steps of an investigation into the proteome. With Dr. Lord, our lab was the first to launch an organic acids test sensitive enough to pick up subtle nutrient perturbations correctible with diet and nutrient interventions: insight into the metabolome.
With the launch of DNA analysis of gut bacteria, fungi and parasites, we could look into an individual’s microbiome.
Taking everything together, including looking at toxic compounds, we were, and are, glancing into the exposome.
Andy and Richard, along with many other great folks, including our education team of Cass Nelson Dooley, Betsy Redmond and Terry Pollock, worked overtime providing the scientific rationale for these investigations in the form of a textbook: Laboratory Evaluations in Integrative and Functional Medicine. Published in 2008, with more than 3800 citations, it remains the functional laboratory standard.
The Medical Education department, led by Dr. Lord, knew its work was essential, important, reflecting the company’s mission statement: To improve health worldwide by providing clinical laboratory testing services in the areas of nutrients, toxicants, hormonal imbalances, biotransformation and detoxification, gastrointestinal function, and the microbiome.
I was reading an article the other day lamenting that men in the US are being all feminized due to the politically correct environment in which we apparently exist. But is this the issue? Can we blame politics for hypogonadism? I think not. However, I do agree wholeheartedly that there is a change occurring.
I tell you folks, the feminization of men is biochemically, not politically, mediated.
I am serious. It’s tragic. And as a red-blooded woman who appreciates men being men, I am not happy about it. But if the obesity, cardiovascular disease, diabetes, cancer and autoimmune epidemics aren’t arousing you to action, maybe the terrible and sad fate of testosterone in this inflammatory soup of modern life will.
Superlatives and hyperbole. But it’s all true.
Let’s take a look at that Big Gulp Coke that Sarah Palin promoted so heavily last year as a natural-born right of all Americans. Let’s track the main Coke ingredient, sugar, down through a primary biochemical pathway in the body and see what happens to it.
The short view: Sugar trashes testosterone. Period.
The slightly longer view: The mechanism by which this happens underlies all chronic inflammatory diseases; from the ubiquitous metabolic syndrome to cancer, autoimmunity and cardiovascular disease.
Some behind-the-scenes biochemistry. And a couple of cases outlining what to do about it:
Sugar stimulates insulin (and chronic sugar ingestion keeps this going and going and going). Insulin promotes the genetic upregulation of the desaturase enzymes that convert the too-highly-abundant-in-the-American-diet linoleic acid (vegetable oils -- think fried foods, potato chips) to arachidonic acid (AA). Arachidonic acid is the parent compound of the exquisitely potent pro-inflammatory molecules called 2- and 4-series eicosanoids. These AA-derived eicosanoids are the most fundamental drivers of all things inflammation in the body. Aracidonic acid is cleaved from the lipid membrane for metabolism to eicosanoids by the enzyme phospholipase A2 (PLA2). A main end-product of this cascade of events is prostaglandin E2 (PGE2). PGE2 promotes genetic upregulation of aromatase (CYP19), the enzyme that converts testosterone to estrogen.
So, high sugar = low testosterone= high estrogen: The feminization of men.
What we can do about it. A couple patient cases.
George is a 57 year old male patient of mine who I’ve been seeing for six years. In fact, we just met last week, hence this blog topic. I began to treat George when I was working at Advanced Diagnostic Pain Treatment Center. He arrived on seven heavy-hitting medications ranging from Fentanyl and oxycodone to atenolol and Avodart (BPH). A handful of NSAIDs were thrown in for good measure. We effectively addressed his complaints, including BPH, hypertension, hyperlipidemia and inflammatory arthritis. He tapered off all of his medications. He quit smoking. He lost weight. He’s been doing well for years, except he’s complained of difficulty with abdominal adiposity and poor muscle mass, despite regular weight and cardio workouts. (He just ran a ½ marathon.) His free and total testosterone levels were actually within normal limits, but his total estrogen level was high. So for George, his good lifestyle (and tapering off opioids- which lower T) has kept his T within normal limits, but he’s still converting too much testosterone to estrogen. George lamented that this was due to genetics. That all the men in his family were cursed with the dreaded “man boobs.”
He also had high total estrogen.
And a love of beer.
We started a fairly straight forward plan. We got extra serious about the diet: very low/no grains (sadly, beer is gone right now); high veg and fiber, with good proteins. He periodically achieves ketosis, which he measures himself in urine. We increased his fish oil intake to inhibit desaturase conversion of linoleic acid to arachidonic acid. (We’re trying to stop production of AA-derived PGE2> aromatase> testosterone> estrogen). I added zinc, chrysin and a natural hops-derived aromatase inhibitor. We started a relatively modest dosage of 50mg DHEA per day. His blood sugar tends to run around 100, so I added berberine 500mg daily. (I know this is lower than the 1500mg/day recommended amount for type II diabetes, but we’re fine-tuning here.) His baseline total estrogen was high at 132. After ten weeks of this protocol, his estrogen was 109; fasting blood sugar 93. A nice drop!
Most importantly is that George is reaping the benefits. He reports more muscle definition (he’s now getting compliments from his gym buddies), better energy, reduced body fat and improved libido and performance. He’s gained muscle weight but his clothes fit better. And the dreaded man boobs? Gone. George is thrilled with his results and wants to continue on the plan. He’s empowered. He’s no longer a victim of his genetics.
On the extra-cautious side, we’ve watched his PSA (free and total), and they’re both consistently low.
George’s baseline total estrogen and free testosterone:
George’s follow-up total estrogen (testosterone pending, but it will be higher based on his clinical response):
One more quick case. With George, we had to really get in there with some fine-tuning to drop his total estrogen. On the other hand, Bill (46 years old, normal weight-for-height) came to my office with complaints of inflammatory arthritis, severe seasonal allergies and GI issues (all inflammatory-driven). Secondary issues included fatigue and loss of muscle mass and definition, despite pushing himself through regular work-outs.
Bill’s treatment plan was relatively straight forward. We focused on his chief complaints with the goal of reducing total body inflammation (which drives up PGE2 and increases estrogens; even allergies contribute here). We did so through a hypoallergenic, lower carbohydrate diet; high dose fish oils, gut repair nutrients (including glutamine, licorice, slippery elm); and a few antimicrobial and anti-inflammatory botanicals (which further inhibit production of PGE2). I also added a hops-derived aromatase modulator and a modest 25mg DHEA/daily. Sublingual immunotherapy was used for allergies. Needed nutrients included B12, B complex, vitamin D and magnesium.
Like George, Bill had normal baseline total and free testosterone. This is really important. While you will commonly see low testosterone in your patients, sometimes values are within normal limits, thus it’s essential to look at total estrogen to get the full picture of what’s happening on the inflammatory front.
Bill’s baseline total estrogen, total and free testosterone.
Bill’s follow-up total estrogen, total and free testosterone.
As you can see from Bill’s baseline and follow-up labs, his estrogen is dropping nicely with the protocol. This result correlated with better muscle mass and exercise tolerance. He also shed a few extra pounds, noticeable in the abdominal region. By reducing total inflammation though a number of mechanisms, including lower carbs and sugar, we achieved a significant drop in estrogens.
As clinicians treating the “Low T” hypogonadal epidemic, we need to take a full systems approach. Endocrine disruption happens from all sides. Yes, diet is our greatest leverage point in virtually all disease. But stress will increase testosterone’s conversion to estrogen and reduce total sex hormone availability. And remember- glucocorticoids are diabetogenic. Toxins- especially plastics and all of the “cides”-pesticides, herbicides, insecticides are xenoestrogens. Think about the sexually ambiguous salmon swimming in polluted waters... Of course, numerous medications will deplete testosterone- opioids, statins. Nutrient depletions will contribute and complicate matters- vitamin D repletion alone has been shown to increase testosterone. Gut and liver health: Can your patients’ biotransform and eliminate estrogens?
Finally, testosterone replacement therapy for hypogonadism is all the rage. Lots of media promotion for treating “Low T” but I’ll tell you this: if you do NOT address the underlying inflammation, then a portion of that nice large pool of exogenous testosterone will indeed be driven on to estrogen.
Links for further reading.
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2265.2012.04401.x/abstract;jsessionid=39FE5839B989B83E037C9DA2BF0C6A1F.f03t04?deniedAccessCustomisedMessage=&userIsAuthenticated=false (low grade inflammation and hypogonadism)
http://care.diabetesjournals.org/content/28/7/1636.long (testosterone, blood glucose, mitochondria)
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3173509/ (BPH, testosterone, blood glucose)
http://www.lipidworld.com/content/13/1/6 (GGT, ALT, AST, obesity, CVD CHD DM PLA)
http://www.medscape.com/viewarticle/589222_4 (metsyn ferritin CVD PLA)
http://tae.sagepub.com/content/1/5/207.abstract (low T and metsyn, abnormal lipids)
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3150075/#R12 (CHD stroke subclinical inflammation and Lp-PLA2)
http://www.ncbi.nlm.nih.gov/pubmed/20362028 (PLA2 cancer)
http://www.ncbi.nlm.nih.gov/pubmed/15670148 (PLA autoimmune)
http://www.ncbi.nlm.nih.gov/pubmed/15670148 (A lot about PGE2)
I recently saw a patient who had been diagnosed with idiopathic Parkinson’s disease (PD) in 2000 when she was just 39 years old. Her history was compelling in that everyone in her family was well --no neurological diseases, parents hearty and healthy at 80 -- and she herself was a healthy child. However, she has a daughter with autism.
It turns out this woman and her husband renovated a lead-saturated 100-year-old Chicago house over the course of 10 years -- in the 1990s. She gave birth to her daughter-who was later diagnosed with the neurological condition autism--while in the house in 1998, around the time she first noticed a tremor.
When urine toxic metals were recently assessed in this patient, her lead (Pb) level was extremely high. In fact, it was eleven times the cut-off limit (her result was 22ug/24 hours; the cut-off limit is 2ug/24h hours). This finding suggests that she has a significant, long-time body burden of lead.
Yes, lead toxicity is a contributing cause of PD and has been implicated in autism.
In PD, lead has been shown to directly damage dopaminergic neurons and contribute to CNS oxidative stress. (In autism, the issue seems to be, in addition to CNS oxidative damage, compromised removal (biotransformation) of lead due to lesions in glutathione conjugation and methylation- more on that below.)
There are currently about one million individuals diagnosed with PD in the US; as with most neurological diseases, its numbers are rising rather rapidly. Diagnosis is made only after the disease shows up clinically— that is, after 60-80% of dopaminergic neurons in the substantia nigra are lost. There are no great biomarkers to conclusively identify disease before this late stage, BUT, if we think about it from a functional/systems medicine perspective, early clues abound.
There is no smoking gun genetic mutation in PD. Yes, there are some genetic mutations that increase risk, (here’s a recent free full text published by PLOS genetics and written by 23 & Me), but having a PD-associated mutation and developing PD isn’t a slam dunk. It’s now well accepted that Parkinson’s Disease, like most complex, chronic conditions is largely caused by chronic environmental exposures, including pesticides, herbicides, toxic metals and the like.
Understanding the toxic connection, it becomes urgent that we regularly evaluate toxin exposures in our patients by taking a careful environmental exposure history and using appropriate labs- such as urine and/or blood toxic metals. When we find concerns, we need to address them immediately by removing the exposure sources and treating the patient, ideally BEFORE they develop clinically apparent complications such as PD.
When I was completing postdoctoral training at a clinical laboratory, we conducted an informal “data mining” project, looking at nutrient imbalances and the presence of toxins in individuals diagnosed with Parkinson’s disease. I was struck by consistent patterns of imbalances. For instance, of the 32 individuals diagnosed with PD who had metals tested, over 60% had higher-than-average to very high lead levels. Additionally, 12 people had higher mercury and 11 had higher aluminum levels.
Of the 10 individuals with PD who had homocysteine levels assessed, all were on the higher side, ranging from 8.7 to 22.6. Six of the 10 individuals with PD had frankly elevated homocysteine. By comparison, a control group of six adults not diagnosed with PD, the homocysteine range was a much lower 5.4 to 9.3.
Incidentally, the highest homocysteine level (22.4) was in the same individual who had the highest lead level.
Methylation and sulfuration: I know you know it by heart…
There are a few ways to think about these data
BUT….We certainly cannot take PD patients off L-dopa. It’s a critical treatment given the damaged dopaminergic neurons.
Our focus must be around carefully supporting normal methylation/sulfuration in this population. Anyone practicing functional medicine knows the drill: folate, B12, betaine and glutathione or the glutathione precursor n-acetylcysteine.
The dance for us as clinicians in treating the individual with PD is to normalize/assist in the removal of toxins by addressing methylation and sulfuration, but not boost methylation so aggressively that we promote excess metabolism of L-dopa. (When PD patients are given the COMT cofactor s-adenosylmethionine, it worsens symptoms.) It is therefore imperative that we monitor homocysteine, toxin levels and clinical symptoms when implementing this approach. Make sense?
Understand that while addressing methylation/sulfuration in the PD population is an essential component to a functional/systems approach, it’s only one area in a much larger systems approach. And as with autism, the earlier we start, the better the outcome. Ideally, we’re preventing the disease by getting there before it starts…….
Living in an old house is the most common cause of lead exposure in the US. And it’s not just renovating an old house. Just generating airborne lead-ladened paint particles from opening and closing windows is a huge exposure source, and inhalation is the most efficient route of lead absorption. But GI absorption is significant as well. Crops grown in lead-exposed soil (think soil saturated with leaded gas) bioaccumulate the toxin. We can use the bioaccumulation phenomenon to remediate soil, but just don’t eat those plants!
We can also find lead in common household items- such as the ceramics we eat on- especially those made in China. I recommend patients purchase at-home lead test kits for testing. I like 3M Lead Check sticks. Get them at Amazon.
A patient recently emailed this: A positive finding for lead on a ceramic dinner plate.
Blood lead cut-off levels have been lowered and lowered over time. This is a figure borrowed from the CDC. See Lead, No Safe Exposure Levels.
It’s been a busy, productive and rather amazing Fall. Lots to write about. In celebration of the gorgeous New England weather, I’ve been spending much time on my bike the last couple of months, and it feels great!
Riding on Ridgefield back roads. Nothing beats it.
I just returned from speaking at the Nutri Advanced conference in London where Drs. Jeff Bland, Andrea Girman and Joe Pizzorno also presented. It was a great conference—the largest yet. UK is embracing Functional Medicine. And London was also absolutely lovely the week I was there.
The Millennium bridge, crossing the Thames from St. Paul’s to the Tate Modern. With Dr. Andrea Girman. We just saw the play Light Princess- words and music by Tori Amos. Amazing!
As always, I was thrilled to hear Dr. Bland speak. A founding father of Functional Medicine (he coined the term), his words nourish intellectually and also spiritually. Listening to him feels like receiving transmission from a Zen master who happens to have a PhD in nutritional biochemistry.
Jeff presented on epigenetics, that is, the way in which environment can shape our DNA expression and determine whether or not we get a particular disease.
So, just because you have the DNA associated with a particular disease doesn’t mean you’re getting the disease. This is such an empowering statement. I’ll say it again: Just because you have the DNA, doesn’t mean you’re getting the disease.
Jeff provided a potent example of this truth: The BRCA1 and BRCA2 mutations are very closely associated with breast and ovarian cancer. Indeed, if you have a BRCA mutation, your lifetime risk of developing breast cancer is greater than 80%. Recall that Angelina Jolie—who has the BRCA1 mutation--just had a double mastectomy to significantly reduce her risk of developing breast cancer.
If you have a BRCA mutation, it seems that you’re going to get cancer, regardless of your health choices, doesn’t it? I certainly admire Angelina’s courage to reduce her risk.
However, if we look into the history of the BRCA mutations and cancer risk, we find that before 1940, the risk was 24% with the BRCA mutation, not 82%, as it is today. Clearly, environment is playing a big role in the development of breast cancer even with the BRCA mutations.
What’s going on?
Dr. Mary King, the scientist who discovered the BRCA mutations, recognized the environmental influence on cancer risk. She specifically cited adolescent obesity, lack of exercise and early age of menarche as factors in the rise of BRCA-associated cancers.
I began thinking about the BRCA mutations and environmental influence in 2007 when I was working at Metametrix Laboratory. My attention was on the influence of fatty acids on hormone-sensitive cancers. A big question for me was: What does a functioning BRCA protein do in the body? As I was drilling down into the literature, I made a fascinating discovery that I rarely see discussed. This discovery gave me a clear treatment direction on how we might reduce risk for individuals with the BRCA mutation.
What does a normally functioning BRCA protein do in the body?
Functioning BRCA proteins are huge, complex structures involved in a good deal of regulatory activity in the body. They are recognized as tumor suppressor genes; they repair DNA.
A lesser recognized BRCA1 role has to do with its ability to potently down-regulate (inhibit) the body’s production of the enzyme aromatase.
What does aromatase do in the body, and why do we want to inhibit it?
Aromatase is the enzyme involved in making estrogen. And in this particular context, estrogen promotes cancers like breast and ovarian. You’ve likely heard of aromatase inhibitors -- drugs that are commonly used to treat these cancers. (Note that estrogen has many essential, healthy, anti-inflammatory roles as well; but that’s for another discussion.)
Not surprisingly, if you look at aromatase in those with the BRCA1 mutation, it’s a lot higher than it should be. So there’s a lot more estrogen than there should be -- hence the cancer risk.
Back to the fatty acid story.
When I discovered the BRCA1/aromatase link, I also learned that the pro-inflammatory fatty acid, called arachidonic acid, makes a compound called PGE2. PGE2 inhibits the good deeds of a normal BRCA1 and promotes aggressive aromatase expression. This results in lots of estrogen production and increased breast and ovarian cancer risk.
In other words, PGE2, from arachidonic acid, can behave like a BRCA1 mutation.
Arachidonic acid (AA) is an omega 6 fatty acid. Some AA is essential for a normal immune response; but anyone eating a typical diabetogenic diet — nutrient-void, too much sugar, simple carb and inflammatory omega 6 fats -- is making way too much arachidonic acid (and therefore PGE2). Globally — not just in the West -- most of us eat this unhealthy diet.
Therefore, one of the most fundamental ways we combat breast and ovarian cancer is by eating a healthy, anti-inflammatory diet. Those with the BRCA mutation? All the more necessary, even urgent, is the anti-inflammatory diet.
Returning to Dr. King’s observations, everything that she suggests will reduce excess or toxic estrogen exposures: More exercise, less obesity (starting early), better diets, later menarche. And of course, all of these are known to reduce cancer risk. (Early menarche is influenced by early estrogen exposures. Therefore, if you want to influence your daughter’s menarche, these changes should be made even before you conceive her, but certainly in early childhood.)
I want this blog to feel empowering for those with the BRCA mutations, or those concerned about hormone-sensitive cancers. The reality is there is much we can do to minimize the impact of estrogens on the body. Any good integrative doctor can come up with a robust to-do list. The fact is, many, many botanicals can interfere with the production of PGE2. If we pack our body full of the good omega 3 fatty acids, also, we will inhibit PGE2 availability. And as I mentioned, perhaps the most powerful factor is an anti-inflammatory eating plan.
Here are just a few of my anti-inflammatory/estrogen modulating/cancer-protective favorites:
What are yours?
Gluten Free and Thyroid Disease Free: An Update on Lilly
The last time we met, Dear Reader, before I high-tailed it on out of the blogosphere for the summer, I introduced you to Lilly and her thyroid woes. Below are her original thyroid studies that she obtained from her primary care doctor and brought to our first meeting. Lilly did not want her endgame to be levothyroxine for life. She sought a second opinion.
June, 2013. Lilly’s baseline thyroid laboratory results demonstrated autoimmune hypothyroidism (also called Hashimoto thyroiditis).
What do these results mean?
As I said in my original post, Lilly’s elevated TSH (thyroid stimulating hormone) demonstrated that her body was working awfully hard trying to get her thyroid to do its job. And the thyroid peroxidase antibody elevation --albeit mild—revealed that Lilly’s own immune system was attacking her thyroid gland. Certainly these results were a big piece of the fatigue puzzle.
Why was Lilly’s immune system attacking her thyroid gland? I must admit, I was pretty certain gluten was involved. I wrote in my post:
It is well-documented that autoimmune hypothyroidism is associated with gluten intolerance. Indeed, celiac disease is associated with myriad autoimmune conditions, including type 1 diabetes, lupus, pernicious anemia, autoimmune hepatitis, Sjogren’s syndrome, Raynaud’s syndrome, myasthenia gravis, and autoimmune hypothyroidism.
Also recall, Dear Reader, that Lilly was entirely loath to remove gluten—her nightly crusty bread with olive oil-- from her diet. Lilly thought the whole gluten-free epidemic was another overhyped phase. (Who can blame her?) She said: “I was tested for celiac disease eight years ago and I am negative. I don’t have a gluten issue.”
I didn’t debate. But I knew that my laboratory investigations with her would include looking for gluten reactivity in its many forms. Here is what I found:
June, 2013. This lab result demonstrates that Lilly’s immune system was reacting to gluten. She didn’t have celiac -- those tests were negative -- but she was definitely sensitive to gluten.
So what did we do?
The lab result was enough to convince Lilly to stop gluten for a while. We negotiated an eight week trial. She understood that it was possible that gluten could be impacting her thyroid. She was 100% motivated to abstain from it entirely for the agreed upon time.
Not surprisingly, Lilly had micronutrient deficiencies as well, including B12 and magnesium. Again, even though she didn’t have celiac disease, it looked like her body was having a hard time absorbing the nutrients it needed. While this is extremely common and well-documented in celiac, it’s less-recognized in gluten sensitivity.
Our treatment plan was straightforward: Avoid gluten, take B12 and minerals. I also ordered a thyroid-centric supplement (containing the nutrients selenium, zinc and tyrosine, designed to support thyroid function) that Lilly took for a little while but stopped when she ran out.
When I chatted with Lilly in July, she told me her gut was better. Gut? Lilly didn’t report any gut issues at our first meeting. It’s interesting how we can identify issues we have tolerated – sometimes for years --only after they’re no longer there! She also said her energy was much, much better. We both knew she was on the right track.
In August, 2013, eight weeks after she started the supplements, Lilly had follow-up testing:
August, 2013. Just eight weeks after the start of going gluten free, Lilly’s thyroid function tests were normal. Her TSH and her antibodies were no longer elevated. Good job, Lilly -- No medication needed!
In functional medicine, we like to see TSH less than 2.00. Lilly’s is almost, but not quite, there. I suggested to Lilly that she restart the thyroid supplement I originally prescribed to see if we can get her into optimal range. I also suggested we continue with the gluten free plan, seeing that she’s doing so well.
Lilly responded immediately, agreeing to take the thyroid support, and proclaiming:
“No gluten since we started and no desire to have any ever again :-)”
Gluten Free: Cliché or Catastrophe?
What do Al Roker, country singer Josh Turner and pregnant “celebutant” Kim Kardashian have in common? If you head over to www.glutenista.com, you will learn that they are all eating gluten free. As is Lady Gaga. She’s apparently “gluten free by choice.”
Lady Gaga: Gluten free by choice.
Wheat Belly. Grain Brain. Gluten Free For Dummies.
A search on the word “gluten” at www.amazon.com under “books” yields 5,569 hits.
A search on the word “gluten” at PubMed (US National Library of Medicine) yields 10,154 hits. (Granted, not all hits are relevant, but a cursory review of the first 20 research abstracts suggests that about 17 are related to gluten-influenced conditions. We can infer that the majority of those 10,154 hits are probably relevant to our topic.)
It was into this fever-pitched, Justin Bieber-styled cultural maelstrom that a lovely woman whom I’ll call Lilly came to see me recently. Lilly’s main complaint was fatigue. She’s bright, and a highly successful business woman in the health food industry. I’ve known her for years. She has seen trends come and go over her 20+ years in the business, from wearing EMF deflecting plastic badges to growing fungal super foods in the coat closet. It’s from this seasoned vantage point that she evaluates many of the über trends in her business.
And Lilly enjoys her crusty bread with extra virgin olive oil on most nights.
She forwarded her recent laboratory data to me prior to our visit. I popped the PDF open and saw this:
Lilly’s recent thyroid laboratory results demonstrate autoimmune hypothyroidism.
Her TSH (thyroid stimulating hormone) was working awfully hard trying to get her thyroid to do its job. And the thyroid peroxidase antibody elevation --albeit mild—revealed that her own immune system was attacking her thyroid gland. Certainly these results were big piece of the fatigue puzzle. But the question is why?
It is well-documented that autoimmune hypothyroidism is associated with gluten intolerance. Indeed, celiac disease is associated with myriad autoimmune conditions, including type 1 diabetes, lupus, pernicious anemia, autoimmune hepatitis, Sjogren syndrome, Raynaud syndrome, myasthenia gravis, and autoimmune hypothyroidism.
I knew immediately that my laboratory investigations with her would include looking for gluten reactivity in its many forms.
When I mentioned this to her, she replied confidently, “I was tested for celiac disease eight years ago and I am negative. I don’t have a gluten issue.”
I didn’t debate. I just ordered the tests.
Who can blame Lilly’s skepticism? Any iconoclast worth his or her salt must be reaching for the Wonder Bread and Budweiser on principle alone. Gluten-free is everywhere. It’s ubiquitous. It can get obnoxious.
But the unfortunate reality is that the incidence of adult-onset celiac disease is rising, rising, rising. In Minnesota alone, the incidence of celiac disease tripled in the last 10 years. And the research around gluten sensitivity-- reactivity to gluten with or without accompanying celiac genetics--is also rising.
Thus, even if Lilly tested negative eight years ago, and doesn’t have the celiac genetics-- it doesn’t mean that gluten hasn’t become a problem for her since then.
Non-celiac gluten sensitivity (GS) has been associated with neurodevelopmental and neurodegenerative conditions, including autism, dementia and ataxia. GS has also been associated with schizophrenia. Indeed, in one study, maternal consumption of gliadin (a component of gluten) and subsequent production of IgG anti-gliadin antibodies was associated with increased risk of psychosis in exposed offspring. In other words- if mom develops an immune response to gluten that results in a rise in IgG anti-gliadin antibodies while pregnant, those IgG antibodies can cross the placental barrier and potentially damage baby’s neurological development. Scary stuff.
It’s an awkward alliance that I and so many of my colleagues find ourselves in with the Kardashians, the Rokers and the Gagas of the world. Integrative physicians and practitioners are, by nature, iconoclastic. I can assure you however, that long after the lights fade on the gluten-free fad, and the Amazon hits dwindle, the gluten-free dietary prescription will continue as it’s needed.
Lilly’s preliminary lab results just came in. Her IgG to gluten is elevated, and probably has something to do with her thyroid issues.
ALMOST FINISHED, by J.K. Bowen-Jones
June 10, 2013 - I want to introduce you to an exquisite book that I've been reading. It's called Almost Finished. It's a memoir by Justine Bowen-Jones. Justine lost her father to pancreatic cancer when she was six years old. Before he died, her father-- poet and English professor Jim Bowen--wrote her letters to accompany her through her life. This book contains his letters and her responses.
I was having a very "bullet point" kind of a day. You know: a million things to do, strong focus, move through the list, tick things off. All very constructive and emotionally void. I had 15 minutes before a meeting, so I grabbed Justine's book for a quick glance, as it had just arrived at my office.
I didn't anticipate the beauty of the book stopping me short. First, the feeling of cognitive dissonance: this book directly challenges in "bullet point" mind frame. It simply can’t be read from that place. The book won, and my day fell away from around me. Here are two very brief excerpts.
Sometimes Jim writes in poetry, as when he says to Justine;
It's not that I feel bad about it
in a common sort of way,
That you will grow old without me;
More that I will miss the ways
you will show me October.
Watching green leaves turn red again,
feeling the touch of the first snow falling.
Softly under the arc of the black street lamp
quietly standing outside
Your bedroom's frosted window,
Hearing you breathe in the warm room air
And at 12 years old, Justine writes back to him,
It's not that I feel bad,
in a common sort of way
that I have grown old,
The dances in the rain,
And the training wheels
I no longer need,
As I look in the frosted window
Of an empty house,
Standing in a place where you once stood.
Almost Finished.by J.K. Bowen-Jones is available on amazon.
Spring has sprung, the flowers are out, and if you’re not planning and planting your garden, stake out your local farmers’ markets. Of the myriad reasons to do so, chances are extremely high that you need some potassium. 93% of us do, according to the National Health and Nutrition Examination Survey (NHANES). No better way to get it than lots of fresh fruits and veggies. (Or avocado and coconut water -- my two favorite sources.)
For you skimmers, I’ll skip to the punchline of this ENL: If we had to pick one single, easy fix for the impending 47,000,000,000,000 US dollar global health care crisis it would be INCREASE FOOD SOURCE POTASSIUM. That’s it. We’d shave 20-30% right off the top of that debt. Done. And there would be a trickle-down effect of a healthy diet beyond that immediate savings, too.
Potassium giveth ...
Sodium taketh away...
Tell Me More….
Most famously, potassium works with its little brother sodium in a delicate ratio, getting pumped in and out of cells. It’s a part of the behind-the-scenes battery using energy (ATP) to generate the pulse of life. Thinking, feeling, heart beating, moving, tasting, breathing and on and on. Potassium is one of the most fundamental players in the game. Any activity requires the action potential that potassium generates as it is being pumped around.
For sake of simplicity, I’ll call the sodium/potassium/ATPase pump The Battery of Life.
Historically, potassium was so ubiquitous in the food supply, deficiency through diet was rare. In fact, look at a Daily Recommended Intake (DRI) table for minerals and you won’t see potassium listed! (You don’t see your DRI for oxygen, either.)
But humans have a way of messing with the most fundamental rules of life, don’t we?
If you’re eating processed foods with any regularity, you’re ingesting too much salt and too little potassium. You’re messing with the Battery of Life. Here’s how bad it is: In the US, according to NHANES, we’re ingesting almost 250% of the DRI of sodium (men: 280%, women: 208%) and only 7% of the recommended amount of potassium.
Way too much, way too little, and way too imbalanced.
Humans are the only mammals to negatively flip the intake of sodium and potassium.
What are the ramifications of this flip?
When we were writing the Elements chapter from Laboratory Evaluations in Integrative and Functional Medicine, we came up with a novel idea: Let’s create a table of the association between essential element deficiencies and top causes of death in the US. We found that potassium deficiency was associated with heart disease, cerebrovascular disease (stroke) and essential hypertension. (What we feel as symptoms of deficiency are vague and widespread but could include muscle cramps, fatigue, slower reflexes.)
Potassium deficiency is associated with the top causes of death worldwide, including hypertension, stroke and heart disease.
About 25% of deaths globally are from heart disease and stroke alone, almost 14 million deaths annually. If we were potassium-replete, especially via fruits and veggies, how much could this number be reduced? How much money could be saved? I remember researching the global cost of hypertension and it alone was pegged at 10,000,000,000,000 US dollars annually. Hence my thesis from second paragraph above that potassium repletion through dietary intake could shave 20-30% off the impending 47 trillion US dollar global medical bill.
A note to a small subset of readers: Yeah, I know, correlation isn’t causation. It seems a pretty tight relationship to me, though. Better still: If we get our potassium from foods, that shift alone will improve morbidity and mortality.
So, in summary, increase potassium-containing foods. A lot. Reduce processed foods. A lot. It’s an easy fix to a longer and healthier life and ultimately will save a lot of money.
Part Two: Assessing potassium and sodium status
This April I was honored to be invited to lecture on allergic disease at the annual Orthomolecular Conference in Toronto, Canada (photos below). As with most good conferences, there are usually a couple “practice changers”; that is, ideas so relevant that they move one beyond just intellectual stimulation and into immediate action and change. For me, one of this year’s came from a lecture delivered by Saul Pilar, MD, a clinician from Vancouver, BC.
Dr Pilar’s simple but powerful take-home message? Measure 24-hour urine sodium (Na+) and potassium (K+) levels in yourself and your patients.
Ruling out confounding factors like Na+/K+-altering diseases and medication effects, urine Na+ and K+ reflect what we eat. Your ratio should be in the vicinity of 1:4 sodium to potassium (1500mg/day Na+ and 4700mg/day K+). And if it’s not? Too many processed foods, too little whole foods. Easy measure, easy fix. (Note: Depending on the reference, there is some disagreement around optimal Na+ and K+ intake, but generally speaking, ballpark is 1:3 to 1:5.)
If you’re wincing at the idea of an onerous 24-hour urine collection, a cursory search of the literature reveals that we can probably get away with this “gold standard” measurement in a 12-hour collection. This suggests that an overnight collection is adequate, too. And this test is routinely insurance-covered.
Generally speaking with regard to minerals, we want to see what’s going on inside the cell. This is usually accomplished by a red blood cell mineral assessment. Looking at intracellular potassium is a good thing, no doubt. However, given the gross imbalance in dietary intake in the case of sodium and potassium, the urine test is best.
Serum sodium and potassium are also routinely assessed; but these levels are so tightly regulated by the body, that you’ll rarely see a nutrient-induced abnormality. If K+ is abnormal in serum, evaluation for causes beyond the diet is essential.
91-year-old Prince Phillip is in the house!
And you can get a blurry glimpse of the proof in this photo. (Arrow provided for your viewing convenience.) Prince Phillip, Queen Elizabeth’s hubby, showed up at the Fairmont Royal York Hotel in Toronto, home of this year’s Orthomolecular Conference. Was he secretly hoping to catch some of the Conference? Maybe... he was in the room right next door!
Prince Phillip’s possee in the Royal York.
For all you Royal-ophiles, his full name is His Royal Highness The Prince Philip, Duke of Edinburgh, Earl of Merioneth and Baron Greenwich, KG (Knight of the Garter), KT (Knight of the Thistle), OM (Order of Merit), GBE (Knight Grand Cross of the Order of the British Empire), AC (Companion of the Order of Australia), QSO (Companion of The Queen’s Service Order), PC (Privy Counsellor). And we thought some integrative physicians have lengthy professional designations….
Also at the conference was Dr. Nicolas Gonzalez talking about Dr. John Beard, pictured on the screen. Dr Beard developed the trophoblastic theory of cancer and pioneered the use of pancreatic enzyme therapy. Very interesting.
Dr Lustig presented on sugar toxicity. Great lecture. See his video, “Sugar, the Bitter Truth,” on YouTube.
Dr. Kara Fitzgerald attended the Nutra India Summit in March.
It was an amazing experience. I was impressed with the level of scholarship and scientific inquiry. Very compelling research was presented on oligosaccharides (prebiotics), probiotics and fermented foods. There were meetings on integrating pharma and nutra. And it all took place against this wonderful backdrop that is Ayurveda, India’s 5000 year old system of medicine.
Dr. Fitzgerald speaks at summit.
Dr. V. Prakash, chairman of Nutra Summit, reminded the audience that the safety studies of today are sorely inadequate when compared to the rules of Ayurveda, where safety and efficacy of a compound require no less than a century of proof before it may enter circulation. Seems hard to believe; but if the system itself is 5K years old, those substances both safe and efficacious will indeed reveal themselves.
Jain temple seen on Dr. Fitzgerald's visit.
Unfortunately, along with India’s meteoric economic development comes the dark shadow of rising chronic disease, which has supplanted infectious disease as the top cause of mortality in India.
Dr. Fitzgerald with panel at summit.
India has one of the highest rates of diabetes world-wide (and it cuts across economic lines). In fact, data from Mumbai showed that a full 95% of its inhabitants had a least one metabolic syndrome biomarker, and about 80% of Mumbaikars are overweight. Food is more plentiful now, especially the fast, fried, simple carbohydrate variety….
An ox pulls a cart through the local streets.
I was pleased to see the same calling towards wellness, however, in my colleagues at the Nutra Summit. Everyone,it seems, is deeply committed to shifting the medical paradigm.
I will return to India in June to present a day-long Introduction to Functional Medicine: Focus on Case Studies.
Dr. Fitzgerald plans to return to India in June.
Gastrointestinal biofilms are an important topic, and those comprised of pathogenic microbes are getting much well-deserved attention in the integrative medical community. However, in keeping with the sIgA topic, I want to give a shout-out to commensal biofilms, which are vital to GI health and deserve similar attention.
Biofilms are everywhere, allowing bacteria to survive -- good or bad. They are found at the solid-liquid interface in most environments. Indeed, dental plaque is a biofilm, as is the slime on an icky bathtub. Biofilms are comprised of bacteria (and/or other microbes) and an extracellular matrix of excreted polymeric polysaccharides.
Simply put: bugs + goo = slime (biofilm).
Slime is nothing to joke about! Biofilms allow pathogenic organisms to be antibiotic resistant, up to 1000-fold by one estimate. But biofilms comprised of commensals can be our friends, modulating our immune response, supporting GI integrity and reducing inflammation.
Research on commensal GI biofilm shows that E. coli , bifidobacteria and L. reuteri are apparently efficient producers, with the former mediated by sIgA and mucin. Research suggests commensal biofilms may be anti-inflammatory, modulate cytokine production and crowd out pathogenic biofilms.
One study in rats with human-type flora showed improved bifidobacteria biofilm, mucus thickness, villous height, crypt depth, and mucin-producing goblet cell numbers when supplemented with inulin-type fructans. How cool is that?
How can we tell if our GI commensal biofilm is healthy?
On a stool test, I would be concerned if I didn’t see enough bifidobacteria, lactobacillus, commensal E. coli or sIgA. Glutamine, vitamin A and S. boulardii support sIgA production; whereas the inulin mentioned above, plus probiotic supplementation, will help facilitate commensal bacterial growth. Finally, treating inflammation and minimizing unwarranted antibiotic use should also benefit biofilm status.
I think we can say that biofilms are little ecosystems unto themselves, where "the sum is greater than the parts.” And when we’re thinking about protection of our all-important GI microbiota, the commensal biofilm is once slimy surface we don’t want to slip away!